ArticleAuthors: Pham, Thi Dau; Le, Thu Ha; Le, Huu Tuyen; Pham, Thi Thu Huong; Hisato, Iwata (2016)
This study investigated the binding affinity of constitutive androstane receptor (CAR) with its activator, 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl) oxime (CITCO) in order to develop a rapid method for screening of protein-binding compounds. At first, the performance capacity of surface plasmon resonance (SPR) system was confirmed by the interaction of commercial carbonic anhydrate II (CAII) protein and 4-carboxybenzenesulfonamide (CBS) compound. The target protein, ligand binding domain of human CAR (hCAR), was optimally immobilized on SPR sensor chip using amine coupling method and then used to detect the interaction with chemical molecules.... 
